Center Spot Filter

Procedure

1)Add a small amount of Propanone into the Mortar. Place a handful of nettle leaves into the Mortar
2)Grind the nettles in the propanone until you get a moderate amount of pigmented solution
3)Use the filter paper to filter the solution into a beaker.
4)Take a piece of Chromatography paper and draw a line 10mm from one of the ends. Mark a point in the centre of the line-this will be the pigment spot.
5)Use the micropipette to extract a sample of the solution and then place a drop onto the spot drawn as before. Let the drop dry, and then place a second small drop on the first. Repeat this process 5 times in order to build up a concentrated pigment in the small area.
6)Whilst preparing the pigment spot, pour the solvent into the boiling tube to a depth no more than 15mm.
7)Fold the opposite end of the Chromatography paper and suspend the Chromatography paper in the boiling tube.